Using Gum Arabic in Place of Egg Yolk as Cryoprotectant for Cryopreservation of the Buck Semen

نویسندگان

چکیده

Background: Egg yolk (EY) is well known to be toxic for buck spermatozoa, which creates restrictions of its use in cryopreservation. Therefore, this study compare the effect different levels gum Arabic (GA) an extender on quality and fertility cryopreserved sperm. Methods: Each ejaculate six bucks was frozen Tris with one concentrations GA contained 3, 6, 9 12 gm/ 100 ml place EY. Control containing 2.5% Result: A percentages total motile sperm (54.92%; P less than 0.05) progressively (26.22%; semen 9% GA. Similar control group, pregnancy rate does inseminated (50.0%) were significantly higher those 6% (8.33%), 3% (0.0%) 12% (0.0%). Semen evaluation similar when replacing EY cryodiluent, after cryopreservation semen. The present shows that high motility acceptable can obtained using cryopreserving

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effect of Different Levels of Egg Yolk on Cryopreservation of Black Bengal Buck Semen in Tris Egg yolk Citrate Fructose Glycerol Extender

This study was conducted to assess the physical and morphological characteristics of fresh, pre-freeze and post-thaw black Bengal buck (Capra hircus) semen processed in Tris-egg yolk-citrate-fructose-glycerol (TEYCFG) extender containing 4 different concentrations (2.5, 5, 7.5 and 10%) of egg-yolk. Ejaculates were collected once a week for eight weeks from six mature bucks (48 ejaculates) using...

متن کامل

Cryopreservation of Bovine Semen in Egg Yolk Based Extenders

................................................................................................................................... ii ACKNOWLEDGEMENTS ........................................................................................................... iv TABLE OF CONTENTS ................................................................................................................ v L...

متن کامل

Comparison of different diluents based on liposomes and egg yolk for ram semen cooling and cryopreservation

Background: Sperm cryopreservation is an important tool for breed improvement, nonetheless, spermatozoids of rams are extremely sensitive to cryopreservation. Aims: The present research was to compare a liposome-based (OptiXcell: OX) diluent, a commercial TRIS-egg yolk (Optidyl: OP) and a citrate egg yolk-based (CY) diluent on ovine semen quality through the cr...

متن کامل

Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier's gazelle (Gazella cuvieri).

Cryopreservation of spermatozoa from endangered species is a valuable tool for genetic management. Previous studies showed the feasibility of cryopreservation of spermatozoa from various endangered gazelles but have also revealed difficulties with available protocols for semen freezing in Cuvier's gazelle (Gazella cuvieri). Experiments were carried out to investigate the effect of (a) 5% or 20%...

متن کامل

Protective role of glutathione in buck semen cryopreservation

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Indian Journal of Animal Research

سال: 2021

ISSN: ['0367-6722', '0976-0555']

DOI: https://doi.org/10.18805/ijar.b-1373